Zoology - Biotechnology - Principles and Processes Question with Solution | TestHub

ZoologyBiotechnology - Principles and ProcessesSeparation and Isolation of DNA FragmentsEasy2 minPYQ_2020
ZoologyEasysingle choice

This segment of DNA has restriction sites I and II which create restriction fragments a, b, and c. Which of the following gels produced by electrophoresis would represent the separation and identity of these fragments?

Options:

Answer:
B
Solution:

In Gel Electrophoresis Nucleic acid molecules are separated by applying an electric field to move the negatively charged molecules through a matrix of agarose or other substances. Shorter molecules move faster and migrate farther than longer ones because shorter molecules migrate more easily through the pores of the gel. This phenomenon is called sieving.
hence c been the longest fragment travel short distance.
a and b are short fragment and nearly equal in size ove farther down.

Stream:NTA_ABHYAS_NEETSubject:ZoologyTopic:Biotechnology - Principles and ProcessesSubtopic:Separation and Isolation of DNA Fragments
⏱ 2mℹ️ Source: PYQ_2020

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