Zoology - Biotechnology - Principles and Processes Question with Solution | TestHub

ZoologyBiotechnology - Principles and ProcessesTools of Recombinant DNA TechnologyMedium2 minPYQ_2025
ZoologyMediumsingle choice


In the above represented plasmid, an alien piece of DNA is inserted at EcoRI site. Which of the following strategies will be chosen to select the recombinant colonies?NEET 2025

Options:

Answer:
C
Solution:

The correct answer is that white-colored colonies will be selected. Since an alien piece of DNA is being inserted at EcoRI site, the gene -galactosidase present here will undergo insertional inactivation. This gene is responsible for producing blue-colored colonies, but since it has been insertionally inactivated, white colored colonies will be produced. Ampicillin and tetracycline resistance genes present in the given DNA will remain intact. Thus, the given DNA will show amp and tet .

Stream:NEETSubject:ZoologyTopic:Biotechnology - Principles and ProcessesSubtopic:Tools of Recombinant DNA Technology
⏱ 2mℹ️ Source: PYQ_2025

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