Zoology - Biotechnology - Principles and Processes Question with Solution | TestHub

PCR stands for a polymerase chain reaction, in this reaction, multiple copies of the gene of interest are synthesized in vitro using two sets of primers and enzyme$\text{DNA}$polymerase. The enzyme extends the primers using the nucleotides provided in the reaction and the genomic$\text{DNA}$as a template. It involves denaturation of the$\text{DNA}$, attachment of primers called annealing and extension by enzyme$\text{DNA}$polymerase.