Zoology - Biotechnology - Principles and Processes Question with Solution | TestHub
ZoologyBiotechnology - Principles and ProcessesSeparation and Isolation of DNA FragmentsEasy2 minPYQ_2019
ZoologyEasysingle choice
DNA's precipitation out of a mixture of biomolecules can be achieved by treatment with:NEET 2019 (All India)
Options:
Answer:
B
Solution:
DNA is highly polarised due to phosphate backbone. This makes it highly soluble in water. Generally, 0.3 M Sodium Acetate is used which neutralizes the negative charge on the PO3- groups on the nucleic acids, making the molecule far less hydrophilic, and therefore much less soluble in water. Then, three volumes of (at least) 95% ethanol is added that removes the hydration shell of H2O molecules around the phosphate.
Stream:NEETSubject:ZoologyTopic:Biotechnology - Principles and ProcessesSubtopic:Separation and Isolation of DNA Fragments
⏱ 2mℹ️ Source: PYQ_2019
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