Zoology - Biotechnology - Principles and Processes Question with Solution | TestHub
pBR322 has restriction sites for many restriction endonucleases. Which of these enzymes acting on pBR322 is not correctly matched with its source?
I. PvuI:Providencia vulgaris
II. ClaI:Caryophanon latum
III. SalI:Streptomyces albus
IV. PstI:Proteus stuarti
Options:
Answer:
Solution:
pBR322 has the following regions:
ori: Region for origin for replication. rop: This region codes for the proteins involved in the replication of plasmid. This region also has recognition sequence for restriction endonuclease Pvu II (Proteus vulgaris). tetR - This region provides resistance against the antibiotic tetracycline. This region also has recognition sequence for restriction endonucleases: Bam H I fromBacillus amyloliquefaciens and Sal I fromStreptomyces albus. ampR - This region provides resistance against the antibiotic ampicillin. This region also has recognition sequence for restriction endonucleases: Pvu I fromProteus vulgaris and Pst I fromProvidencia stuarti.Apart from these, pBR 322 also shows recognition sequence for restriction endonucleases: EcoR I fromEscherichia coli, Hind III fromHaemophilus influenza, Cla I fromCaryophanon latum
