Zoology - Biotechnology - Principles and Processes Question with Solution | TestHub
ZoologyBiotechnology - Principles and ProcessesTools of Recombinant DNA TechnologyEasy2 minPYQ_2020
ZoologyEasysingle choice
If pBR322 cloning vector is cut by PvuI enzyme for the formation of rDNA then which of its marker gene helps in the selection of recombinants?
Options:
Answer:
D
Solution:
pBR 322 has two selectable markers which are amphicillin and tertacyclin resistant genes. Amphicilin resistant gene has two restriction enzyme recognition site, Pvu I and Pst I. If the vector is cut by Pvu I enzyme for formation of rDNA than amphicillin resistant gene becomes inactive due to insertional inactivation.
Stream:NTA_ABHYAS_NEETSubject:ZoologyTopic:Biotechnology - Principles and ProcessesSubtopic:Tools of Recombinant DNA Technology
⏱ 2mℹ️ Source: PYQ_2020
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