Zoology - Biotechnology - Principles and Processes Question with Solution | TestHub
Read the following sentences:-
(a) In elution, the separated bands of DNA are cut out from agarose gel & extracted from the gel piece.
(b)E coil cloning vector pBR322 have several restriction sites, Ori, antibiotic resistance genes & ROP.
(c) The downstream processing and quality control testing vary from product to product.
(d) Competent bacterial cells cannot take up the plasmid.
Options:
Answer:
Solution:
In order to force bacteria to take up the plasmid, the bacterial cells must first be made ‘competent’ to take up DNA. This is done by treating them with a specific concentration of a divalent cation, such as calcium, which increases the efficiency with which DNA enters the bacterium through pores in its cell wall. Recombinant DNA can then be forced into such cells by incubating the cells with recombinant DNA on ice, followed by placing them briefly at 42oC (heat shock) and then putting them back on ice. This enables the bacteria to take up the recombinant DNA.
