Zoology - Biotechnology - Principles and Processes Question with Solution | TestHub
Read the following statements regarding DNA recombinant technology. Identify the statements that are true and false and select the appropriate option.
Selectable marker helps in identifying and eliminating non-transformants and selectively permitting the growth of the transformants.
Plasmids have the ability to replicate within bacterial cells independent of the control of chromosomal DNA making it a suitable cloning vector.
The DNA fragments for isolation can be separated by a technique known as thin-layer chromatography.
A technique where cells are bombarded with high velocity micro-particles of Au or W coated with DNA is known as micro-injection.
| I | II | III | IV | |
| 1 | False | False | True | True |
| 2 | True | True | False | False |
| 3 | True | True | True | True |
| 4 | False | False | True | False |
Options:
Answer:
Solution:
A marker is necessary for the DNA recombinant technology to identify the transformed cell which will have our gene of interest. Plasmids are autonomously replicating circular extra-chromosomal DNA hence they are a very good tool for DNA recombination. When gold or tungsten microparticles are used for transferring the gene of interest into the cell, the technique is called gene gun. Micro-injection is the process of injecting a micro-needle into the nucleus to insert the gene of interest. DNA fragments are separated using the process called AGE- agarose gel electrophoresis.
