Zoology - Biotechnology - Principles and Processes Question with Solution | TestHub
ZoologyBiotechnology - Principles and ProcessesSeparation and Isolation of DNA FragmentsEasy2 minPYQ_2020
ZoologyEasysingle choice
In the process of transformation, divalent ions are used to make the host competent. The function of such ions is to
Options:
Answer:
A
Solution:
In order to force bacteria to take up the plasmid, the bacterial cells must first be made ‘competent’ to take up DNA. This is done by treating them with a specific concentration of a divalent cation, such as calcium, which increases the efficiency with which DNA enters the bacterium through pores in its cell wall. Recombinant DNA can then be forced into such cells by incubating the cells with recombinant DNA on ice, followed by placing them briefly at 42°C (heat shock), and then putting them back on ice. This enables the bacteria to take up the recombinant DNA.
Stream:NTA_ABHYAS_NEETSubject:ZoologyTopic:Biotechnology - Principles and ProcessesSubtopic:Separation and Isolation of DNA Fragments
⏱ 2mℹ️ Source: PYQ_2020
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