Zoology - Biotechnology - Principles and Processes Question with Solution | TestHub

ZoologyBiotechnology - Principles and ProcessesTools of Recombinant DNA TechnologyEasy2 minPYQ_2020
ZoologyEasysingle choice

The colonies of recombinant bacteria appear white in contrast to blue colonies of non-recombinant bacteria because of

Options:

Answer:
C
Solution:

Screenable marker, visual marker or reporter gene generates a product that can be detected. It is used in confirming transformation, determining transformation efficiency and monitoring gene expression. 

The most common example is the lac Z gene of E. coli. Lac Z gene acts as a screenable marker in the presence of X-gal because its expression is readily quantitated by the intensity of the blue colour that is generated.

Lac Z (reporter gene) → β-galactosidase (gene product) →X-gal (substrate) →1. Blue colonies  (non-transformants) 2. White colonies (transformants)

Stream:NTA_ABHYAS_NEETSubject:ZoologyTopic:Biotechnology - Principles and ProcessesSubtopic:Tools of Recombinant DNA Technology
⏱ 2mℹ️ Source: PYQ_2020

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