Zoology - Biotechnology - Principles and Processes Question with Solution | TestHub
The colonies of recombinant bacteria appear white in contrast to blue colonies of non-recombinant bacteria because of
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Screenable marker, visual marker or reporter gene generates a product that can be detected. It is used in confirming transformation, determining transformation efficiency and monitoring gene expression.
The most common example is the lac Z gene of E. coli. Lac Z gene acts as a screenable marker in the presence of X-gal because its expression is readily quantitated by the intensity of the blue colour that is generated.
Lac Z (reporter gene) -galactosidase (gene product) X-gal (substrate) 1. Blue colonies (non-transformants) 2. White colonies (transformants)
