Zoology - Biotechnology - Principles and Processes Question with Solution | TestHub

pBR322 has two restriction sites BamHI (fromBacillus amyloliquefaciens) and SalI (fromStreptomyces albus) on the tetracycline-resistant gene (tetR), which acts as a selectable marker. It also has two restriction sites PstI (from Providenciastuartii) and PvuI (fromProteus vulgaris) on ampicillin-resistant gene (ampR), which also acts as a selectable marker. Hence, these four enzymes can be used. however, the restriction site for PvuII is present on rop (replication of plasmid) region. If used, it will interfere with the process of replication of plasmid.