Zoology - Biotechnology - Principles and Processes Question with Solution | TestHub
ZoologyBiotechnology - Principles and ProcessesTools of Recombinant DNA TechnologyEasy2 minPYQ_2020
ZoologyEasysingle choice
If alien DNA is introduced using Pst I in pBR322 then transformant will grow on:
Options:
Answer:
B
Solution:
TetRgene in pBR322 can be cleaved by Bam I and Sal I, while AmpRgene in pBR322 is cleaved by Pst I and Pvu I. If foreign DNA segment is inserted in Pst I site than ampicillin-resistant gene becomes inactive due to insertional inactivation and transformant can be grown on tetracycline containing medium.
Stream:NTA_ABHYAS_NEETSubject:ZoologyTopic:Biotechnology - Principles and ProcessesSubtopic:Tools of Recombinant DNA Technology
⏱ 2mℹ️ Source: PYQ_2020
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