Zoology - Biotechnology - Principles and Processes Question with Solution | TestHub
Which of following are the steps of genetic engineering?
Options:
Answer:
Solution:
Genetic engineering:It is manipulation of genetic material which can be added or detected through their bases or parts of DNA to introduce these into host organisms and thereby change the phenotype of the host organism.
The steps of genetic engineering are
(i) Isolation of DNA.
(ii) Separation by gel electrophoresis.
(iii) Isolation of the desired DNA fragments.
(iv) Amplification of the gene of interest.
(v) Ligation of the DNA fragment into a vector using DNA Ligase.
(vi) Transfer of recombinant DNA into the host organism.
(vii) Culturing the host cell on a suitable medium on a large scale.
(viii) Extraction of the desired product.
(ix) Downstream processing of the products as finished products is ready for marketing.
