Zoology - Biotechnology - Principles and Processes Question with Solution | TestHub
Assertion: Restriction enzymes cut the strand of DNA to produce sticky ends. Reason : Stickiness of the ends facilitates the action of the enzyme DNA polymerase.
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Restriction enzyme, a type of endonuclease, functions by "inspecting" the length of a DNA sequence. Once it finds a recognition sequence, it binds and cuts each of the two strands of the double helix at specific point leaving single stranded portions at the ends. This results in overhanging stretches called sticky ends. These are named so because they form hydrogen bonds with their complementary counter parts, i.e., they can join similar complementary ends of DNA fragment from some other source with the help of DNA ligase. This stickiness of the ends facilitates the action of the enzyme DNA ligase, not DNA polymerase.
