Zoology - Biotechnology - Principles and Processes Question with Solution | TestHub
ZoologyBiotechnology - Principles and ProcessesSeparation and Isolation of DNA FragmentsEasy2 minPYQ_2021
ZoologyEasysingle choice
During the process of gene amplification using PCR, if a very high temperature is not maintained in the beginning, then which of the following steps of PCR will be affected first?NEET 2021
Options:
Answer:
C
Solution:
Denaturation , annealing- , extension- . Since maximum temperature is required during denaturation, it will be affected the most.
The initial denaturation step is carried out at the beginning of PCR to separate the double-stranded template DNA into single strands so that the primers can bind to the target region and initiate extension.
Stream:NEETSubject:ZoologyTopic:Biotechnology - Principles and ProcessesSubtopic:Separation and Isolation of DNA Fragments
⏱ 2mℹ️ Source: PYQ_2021
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