Zoology - Biotechnology and Its Applications Question with Solution | TestHub
Which of the following statements are correct about genetically engineered insulin?
(i) It was manufactured by an American company Eli Lilly.
(ii) It is produced by the use of appropriate recombinantE. coli clones.
(iii) It was launched on 5th July 1983.
(iv) It is also called bovine insulin and is used for the treatment of diabetes mellitus.
Options:
Answer:
Solution:
The main challenge for the production of insulin using the rDNA technique was getting insulin assembled into a mature form with the removal of С peptide. In 1983, Eli Lilly, an American company, first prepared two DNA sequences corresponding to A and В chains of human insulin and introduced them in plasmids ofEscherichia colito produce insulin chains. Chains A and В were produced separately, extracted and combined by creating disulfide bonds to form human insulin (Humulin).
