Zoology - Biotechnology and Its Applications Question with Solution | TestHub
Assertion : Eli Lily prepared two DNA sequences corresponding to and chain of human insulin and introduced them in the plasmid of E.coli to produce polypeptide chains of insulin. Reason : Chains A and B were produced separately, extracted and combined by creating disulphide bonds to form human insulin.
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Insulin is synthesised as a prohormone which contains an extra stretch called C peptide. This C peptide is not present in the mature insulin as it is removed during maturation. The main challenge for insulin production using rDNA technique was getting insulin assembled into a mature form. In 1983, Eli Lily, an American company, first prepared two DNA sequences corresponding to and chains of human insulin and introduced them in plasmids of . coli to produce insulin chains. Chain A and B were produced separately, extracted and combined by creating disulphide bonds to form human insulin.
