A microscope with a numerical aperture (NA) of 1.25 is used to observe small biological samples with light of wavelength 500 nm . The resolving power of the microscope is influenced by factors such as

Question

A microscope with a numerical aperture (NA) of 1.25 is used to observe small biological samples with light of wavelength 500 nm . The resolving power of the microscope is influenced by factors such as the wavelength, numerical aperture, and immersion medium. Which of the following statements are correct about the resolving power of the microscope?

TestHub · Accepted Answer

Smaller minimum distance means better resolution.

$d = \frac{0.61 λ}{N A}$

$N A = n sin θ$

(A) Shorter wavelength reduces the minimum distance: correct.

(B) Larger numerical aperture reduces it: correct.

(C) Higher-index immersion oil can raise the numerical aperture: correct.

(D) A wavelength of 1000 nm instead of 500 nm worsens resolution: incorrect.

Answer: A, B, C.

Physics - Geometrical Optics Question with Solution | TestHub

Options:(select one or more)

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