Chemistry - PRACTICAL ORGANIC CHEMISTRY Question with Solution | TestHub
Using column chromatography, mixture of two compounds ‘’ and ‘’ was separated. ‘’ eluted first, this indicates ‘’ has
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In chromatography, the retardation factor () is the fraction of an analyte in the mobile phase of a chromatographic system. In planar chromatography in particular, the retardation factor is defined as the ratio of the distance travelled by the centre of a spot to the distance travelled by the solvent front. The more polar spot travels slower, and the less polar spot travels faster. values, on the other hand, are directly related to the rate of movement. The fastest moving spot has the highest value, least polar (fastest moving), and the spot with the lowest value is the most polar (slowest moving). Hence, the compound is strongly adsorbed and has low value.