Chemistry - Biomolecules Question with Solution | TestHub

Substrate(s) $\stackrel{enzymeE}{\to }$Product (P)

Michaelis-Menten mechanism for the enzyme catalyzed reaction is

(Enzyme-substrate complex)

$ES\stackrel{K_b}{\to }P+E$

The rate of reaction is given by the Michaelis-Menten equation:

$r=\frac{K_{b.}\left[E_0][S]\right]}{[S]+K_m}$

Where $[E{]}_0$ = Initial concentration of enzyme and $K_m$= Michaelis constant $=\frac{K_b+K_a^{{}^{\prime }}}{K_a}$

Normally, the substrate remains in large excess relative to the enzyme, so the free substrate concentration at any time is approximately equal to the initial substrate concentration $[S]\approx [S{]}_0$