Biology - Biotechnology and Its Applications Question with Solution | TestHub
Arrange the sequence which results in killing of insects which feed on BT plant.
(A). Midgut paralysis and cells of insect are disrupted.
(B). The insect ingests bacterial spores with cry gene present in the insect resistant transgenic plant.
(C). A combination of high pH and the enzyme proteinase of the insects's midgut, processes crystalline inclusions hydrolytically to release the core toxic fragments.
Options:
Answer:
Solution:
The correct sequence of events leading to the death of an insect feeding on a Bt plant is as follows:
First, the insect feeds on the transgenic plant and ingests the inactive protoxin (B).
Second, the inactive protoxin is converted into an active form of toxin due to the alkaline pH of the midgut, which solubilizes the crystals, and proteinases process it to release the core toxic fragments (C).
Third, the activated toxin binds to the surface of midgut epithelial cells and creates pores that cause cell swelling and lysis, leading to midgut paralysis and eventually the death of the insect (A).
The correct sequence is (B) (C) (A).
Answer: (B) (C) (A)
