Some of the steps involved in the production of humulin are given below. Choose the correct sequence.

(1) Introduction of the recombinant plasmid intoE. coli.
(2) Extraction of a recombinant gene product fromE. coli.
(3) Culturing recombinantE. coliin bioreactors.
(4) Insertion of the human insulin gene into a plasmid.

Question

TestHub · Accepted Answer

Humulin was first produced by an American company Eli Lily. The human insulin synthesizing gene was isolated and introduced into a plasmid. The recombinant plasmid was then introduced into E. coli which was then cultured in a bioreactor. Finally, the product was isolated and purified.

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