In which of the following case, mice will remain alive in Griffith's transforming experiments?

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Transforming Principle (by Frederick Griffith, 1928)

- Frederick Griffith has done experiments on bacteria Streptococcus pneumonia. This bacteria causes pneumonia.

- Griffith has observed the change in the physical form of the bacteria during the experiments.

- Streptococcus pneumonia produces 2 types of colonies :

$1 .$ Smooth and shiny colonies (S - strain or virulent strain) - Bacteria possess mucous

(polysaccharide) coat.

$2 .$ Rough colonies (R - strain) - Bacteria lacks mucous coat.

Step 1	S –strain injected into the mice	Mice die
Step 2	R –strain injected into the mice	Mice live
Step 3	Heat killed S –strain injected into the mice	Mice live
Step 4	Heat killed S –strain + R –strain injected into the mice	Mice die

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