Zoology - Molecular Basis of Inheritance Question with Solution | TestHub
Which of the following statements are false regarding 'Hershey and Chase' experiment, proving DNA is genetic material?
(i) They made use of rats.
(ii) Radioactivity was seen in supernatant for the medium that was infected by phage radiolabeled with32P.
(iii) Used radioisotope to label sulphur with35S in sulphur-containing amino acids.
(iv) Radioactive phages were allowed to attach toE. colibacteria.
Options:
Answer:
Solution:
Martha Chase and Alfred D.Harshey conducted an experiment that proved DNA is the genetic material that is inherited. In their experiment they used radioactive isotope of Sulfurand Phosphoruscontaining bacteriophage T2 (dsDNA) to infect a bacterium,Escherichia coli and found that the phosphorus-containing DNA of the viral particle, not the sulfur-containing protein of the viral coat, enters the host cell and serves the genetic information for viral replication. To conduct the experiment, the bacteriophage and bacteria was blended and constituents were separated by density gradient centrifugation.
