Zoology - Molecular Basis of Inheritance Question with Solution | TestHub

Reverse Transcription Polymerase Chain Reaction (RT-PCR) is a variant of Polymerase Chain Reaction (PCR), a laboratory technique commonly used in molecular biology to generate many copies of a DNA sequence, a process termed "amplification". In RT-PCR, however, an RNA strand is first reverse transcribed into its DNA complement (complementary DNA, or $c$ DNA) using the enzyme reverse transcriptase, and the resulting $c$ DNA is amplified using traditional PCR or Real-Time PCR. Reverse transcription PCR (RT-PCR)is not to be confused with RealTime Polymerase Chain Reaction (Q-PCR/qRTPCR), RNA silencing (also called as posttranscriptional gene silencing PTGS) refers to a family of gene silencing effects by which the expression of one or more genes is down regulated or entirely suppressed by the introduction of an antisense (single stranded) RNA molecule. Transcription is the process of creating a complementary RNA copy of a sequence of DNA.