Zoology - Biotechnology - Principles and Processes Question with Solution | TestHub

Polymerase Chain Reaction (PCR) involves three main steps in each cycle:

Denaturation: The double-stranded DNA is heated to a high temperature (around $94^{\circ }\text{C}$) to break the hydrogen bonds, resulting in two single strands.

Annealing: The temperature is lowered (around $50^{\circ }\text{C}$ to $65^{\circ }\text{C}$) to allow the primers to bind to the complementary sequences on the single-stranded DNA.

Extension: The temperature is raised (around $72^{\circ }\text{C}$) to allow Taq polymerase to synthesize the new DNA strand by adding nucleotides to the primers.

Thus, the correct sequence is Denaturation $\to$ Annealing $\to$ Extension.

Answer: Denaturation $\to$ Annealing $\to$ Extension