Zoology - Biotechnology - Principles and Processes Question with Solution | TestHub
ZoologyBiotechnology - Principles and ProcessesSeparation and Isolation of DNA FragmentsMedium2 minPYQ_2023
ZoologyMediumsingle choice
Main steps in the formation of Recombinant DNA are given below. Arrange these steps in a correct sequence.
A. Insertion of recombinant DNA into the host cell.
B. Cutting of DNA at specific location by restriction enzyme.
C. Isolation of desired DNA fragment.
D. Amplification of gene of interest using PCR.
Choose the correct answer from the options given below:
NEET 2023 (All India)
Options:
Answer:
C
Solution:
The process of forming a recombinant DNA molecule involves the following steps:
- Isolation of gene ot interest or a desired DNA molecule from an organism.
- Restriction digestion of the desired DNA into fragments to insert into the vector. This is carried out by restriction enzymes.
- Isolation of these fragments of DNA (desired fragment)
- The fragments can be separated by gel electrophoresis.
- Amplification of the gene by PCR thermo-cycler.
- Insertion of recombinant DNA into the host cell.
Stream:NEETSubject:ZoologyTopic:Biotechnology - Principles and ProcessesSubtopic:Separation and Isolation of DNA Fragments
⏱ 2mℹ️ Source: PYQ_2023
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