Zoology - Biotechnology - Principles and Processes Question with Solution | TestHub
Given below are two statements: one is labelled as Assertion (A) and the other is labelled as Reason (R).
Assertion (A): Polymerase chain reaction is used in DNA amplification
Reason (R): The ampicillin resistant gene is used as a selectable marker to check transformation
In the light of the above statements, choose the correct answer from the options given below :
NEET 2022 (Phase 1)
Options:
Answer:
Solution:
The polymerase chain reaction (PCR) is an artificial method of the gene amplification by which several copies of the desired DNA segment can be obtained in a short period. This technique was developed by Kary Mullis in 1985. This technique is based on a principle that the DNA undergoes denaturation at high temperature. Selectable markers are used to select for successful transformants from untransformed cells. The ampicillin resistant gene in the plasmid is one of the example of this.
