Plasmid pBR 322 has PstI restriction enzyme site within gene amp R that confers ampicillin resistance. If this enzyme is used for inserting a gene for β -galactoside production and the recombinant pla

Question

Plasmid pBR 322 has PstI restriction enzyme site within gene amp R that confers ampicillin resistance. If this enzyme is used for inserting a gene for β -galactoside production and the recombinant plasmid is inserted in an E. coli strainNEET 2021

TestHub · Accepted Answer

pBR 322 has two antibiotic-resistant genes for ampicillin (ampR) and tetracycline (tetR). Due to the insertion of the gene in the ampR region, insertional inactivation results in the inactivation of the ampicillin gene and hence the organisms become sensitive to this antibiotic and do not grow in the media containing this antibiotic.

Zoology - Biotechnology - Principles and Processes Question with Solution | TestHub

Options:

Doubts & Discussion