Zoology - Biotechnology - Principles and Processes Question with Solution | TestHub

Polymerase chain reaction is an in vitromethod of gene amplification. One cycle of PCR has three steps:

$1.$Heat denaturation in which DNA is heated at 90${\mathrm{°C}}^{}\mathrm{.}$

$2.$Annealing in which the two DNA strands bind to their respective primers at 55${\mathrm{°C}}^{}.$Polymerisation in which the primer elongates at 72${\mathrm{°C.}}^{}$