Zoology - Biotechnology - Principles and Processes Question with Solution | TestHub

The correct order of steps in polymerase chain reaction is denaturation, annealing and extension.

At high temperature hydrogen bonds between nitrogen bases of two strands of DNA breaks and double stranded DNA separates as single strands, this is known as denaturation. Small DNA primers attaches to the template strand and this is known as annealing. Taq polymerase now adds complementary nucleotides to template, this is called as extension.