Match the organism with its related contribution in the field of biotechnology.
Column-I Column-II
$1$ Escherichia coli $I$ $1^{s t}$ rDNA
$2$ Agrobacterium tumefaciens $I I$ $p B R 322$
$3$ Thermus aquaticus $I I I$ Ti plasmid
$4$ Salmonella typhimurium $I V$ Polymerase used in PCR

Question

Match the organism with its related contribution in the field of biotechnology.

	Column-I		Column-II
$1$	Escherichia coli	$I$	$1^{s t}$ rDNA
$2$	Agrobacterium tumefaciens	$I I$	$p B R 322$
$3$	Thermus aquaticus	$I I I$	Ti plasmid
$4$	Salmonella typhimurium	$I V$	Polymerase used in PCR

TestHub · Accepted Answer

1st recombinant DNA was constructed by Cohen and Boyer using the plasmid ofSalmonella typhimurium

pBR322 is a plasmid cloning vector found inE. coli. Taq polymerase is an enzyme obtained from bacteriaThermus aquaticus used in PCR. This enzyme has high-temperature tolerance compared to other polymerases.A. tumefaciensis a known tumor-inducing microbe infecting plants in the roots. Its plasmid (Ti plasmid) has become a useful tool for recombination technology as this microbe originally transfers the Ti gene into the host genome.

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