Zoology - Biotechnology - Principles and Processes Question with Solution | TestHub
ZoologyBiotechnology - Principles and ProcessesSeparation and Isolation of DNA FragmentsEasy2 minPYQ_2020
ZoologyEasysingle choice
The denaturation process in Polymerase Chain Reaction (PCR) does not involve
Options:
Answer:
C
Solution:
PCR stands for Polymerase Chain Reaction. In this reaction, gene (segment of DNA) of interest is synthesised in vitro and multiple copies are generated. The main steps of PCR-
Denaturation - where the double-stranded template DNA is heated to separate it into two single strands.
Annealing - where the temperature is lowered to enable the DNA primers to attach to the template DNA.
Extension - where the temperature is raised and the new strand of DNA is made by the Taq polymerase
enzyme. Thus, option C is correct as it is part of extension step and not denaturation.
Stream:NTA_ABHYAS_NEETSubject:ZoologyTopic:Biotechnology - Principles and ProcessesSubtopic:Separation and Isolation of DNA Fragments
⏱ 2mℹ️ Source: PYQ_2020
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