Zoology - Biotechnology - Principles and Processes Question with Solution | TestHub

PCR is Polymerase chain reaction. This technique is used to form numerous copies of a specific segment of DNA. Thus large quantities of DNA are obtained that are required for various experiments in medicine, molecular biology, forensic biology etc. The steps are:

$1.$Denaturation of the template into single strands. The temperature is 94 degree celsius.

$2.$Annealing of primers to each original strand for new strand synthesis. the temperature is 50-60 degree celsius

$3.$Polymerisation :Extension of new DNA strands from the primers. The temperature is 72 degree celsius.