Zoology - Biotechnology - Principles and Processes Question with Solution | TestHub
ZoologyBiotechnology - Principles and ProcessesTools of Recombinant DNA TechnologyEasy2 minPYQ_2020
ZoologyEasysingle choice
Suppose the gene of interest is inserted at PstI site in pBR322, the resulting plasmid will confer resistance to:
Options:
Answer:
D
Solution:
The ligation of alien DNA can be carried out at a restriction site present in one of the two antibiotic resistance genes in pBR322. For example, you can ligate a foreign DNA at the PstI site of the ampicillin resistance gene in the vector pBR322. The recombinant plasmids will lose ampicillin resistance due to insertion of foreign DNA and vice versa.
Stream:NTA_ABHYAS_NEETSubject:ZoologyTopic:Biotechnology - Principles and ProcessesSubtopic:Tools of Recombinant DNA Technology
⏱ 2mℹ️ Source: PYQ_2020
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