You discovered a novel eukaryotic organism that glows in the dark. You believe this trait is due to a single gene, and you wish to clone the gene. Which of the following strategies is most likely to b

Question

You discovered a novel eukaryotic organism that glows in the dark. You believe this trait is due to a single gene, and you wish to clone the gene. Which of the following strategies is most likely to be successful?

TestHub · Accepted Answer

The eukaryotic organism glows in the dark and this is due to a single gene which needs to be cloned. Hence we will select Eukaryotic organisms. The gene is unidentified and hence for cloning restriction endunucleases cannot be used as these enzymes recognise specific sites only. Hence now we need to transcribe this gene to produce cDNA. Then by using a suitable vector like plasmid vector this cDNA is transformed into the eukaryotic cells and then screening is done to check the colonies whether they glow in dark or not.

Zoology - Biotechnology - Principles and Processes Question with Solution | TestHub

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