Zoology - Biotechnology - Principles and Processes Question with Solution | TestHub
The efficiency of the cell to take up rDNA is increased by
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Divalent cation like calcium increases the permeability of bacterial cell wall for DNA so that the DNA readily enters the cell.
In order to force bacteria to take up the plasmid, the bacterial cells must first be made ‘competent’ to take up DNA. This is done by treating them with a specific concentration of a divalent cation, such as calcium, which increases the efficiency with which DNA enters the bacterium through pores in its cell wall. Recombinant DNA can then be forced into such cells by incubating the cells with recombinant DNA on ice, followed by placing them briefly at 420C (heat shock) and then putting them back on ice. This enables the bacteria to take up the recombinant DNA.
