Zoology - Biotechnology - Principles and Processes Question with Solution | TestHub
Read the following statements and select the correct option.
(I)E.colicloning vector pBR322 have several restriction sites,ori, antibiotic resistance genes androp.
(II) Competent bacterial cells cannot take up foreign DNA.
(III) In elution, the separated bands of DNA are cut out from agarose gel and extracted from the gel piece.
(IV) The downstream processing and quality control testing vary from product to product.
Options:
Answer:
Solution:
Vector pBR 322 have 8 restriction enzyme recognition site, ori, antibiotic resistant genes (Amphicilin and tertacyclin) which function as selectable marker and rop. Competent bacterial cell can take up foregin DNA. The extraction of DNA from gel block after gel electrophoresis is called as elution.
