The presence of a chromogenic substrate gives blue coloured colonies of bacteria. Insertional inactivation would stop production of which of the following enzyme such that colonies do not produce any

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The presence of a chromogenic substrate gives blue coloured colonies of bacteria. Insertional inactivation would stop production of which of the following enzyme such that colonies do not produce any colour.

TestHub · Accepted Answer

In PUC plasmid, Lac Z gene is used as a selectable marker. If a transformant is grown in a medium having chromogenic substrate it produces enzyme Beta-galactosidase due to which blue coloured colonies are produced. Insertion of foreign DNA segment in Lac Z gene fails to produces enzyme Beta-galactosidase as a result colourless colonies are produced.

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