Zoology - Biotechnology - Principles and Processes Question with Solution | TestHub

In annealing, primers anneal to the DNA template, to provide a site for initiation of polymerisation to enzyme Taq polymerase. Before this denaturation of DNA takes place in which two strands of DNA separate from each other at high temperature of $92-95^{\circ }\mathrm{C}$. Extensions is last stage of PCR, in which Taq polymerase enzyme adds nucleotides to annealed primer and synthesise new strand.