Zoology - Biotechnology - Principles and Processes Question with Solution | TestHub

When genomic DNA extracted from any tissue of a plant or animal species is digested with a restriction enzyme, it is cleaved into segments. The segments of different sizes can be separated through gel electrophoresis. Gel electrophoresis involves movement of fragments or molecules on the basis if molecule size and shape under the influence of electric field. Endonucleases are enzymes that produce internal cuts called cleavage in DNA molecules. It cleaves DNA only within or near recognition sites, which have specific base sequences, such endonucleases are known as restriction endonucleases. The selected vector is cut open with a restriction enzyme that has a unique target site located in the sequence, where the DNA insert should be integrated to facilitate the selection of recombinant DNA.