Zoology - Biotechnology and Its Applications Question with Solution | TestHub
The figure below shows three steps (A, B, C) of Polymerase Chain Reaction (PCR). Select the option giving correct identification together with what it represents ?
Options:
Answer:
Solution:
PCR is a technique for enzymatically replicating DNA without using a living organism such as E. coli or yeast. It is commonly used in medical and biological research labs for a variety of tasks like detection of hereditary diseases identification of genetic fingerprints etc.
The correct steps shown in the above figure are:
A - Denaturation at a temperature of about 94oto 98oC. During the denaturation, the double strand melts open to single stranded DNA, and all enzymatic reactions stop.
B - Annealing (binding of DNA primer to the separated strands. Occurs at 50oto 65oCelsius, which is lower than the optimal temperature of the DNA polymerases)
C - Extension or elongation of the stands using the DNA primer with heat-sable DNA polymerases, most frequently Taq (Thermus aquaticus) at 72oC.
