Chemistry - Biomolecules Question with Solution | TestHub
Among the statements given below, which statement/s is/are correct regarding the relationship between enzyme and substrate?
I. Above a certain concentration of substrate, an enzyme reaches its maximum rate of reaction
II. Increasing the substrate concentration to a higher level doesn't reverse the effects of a competitive inhibitor.
III. At high substrate concentration, a non-competitive inhibitor no longer affects the enzyme activity.
IV. The higher the concentration of substrate the faster an enzyme can catalyse a reaction.
Options:
Answer:
Solution:
Substrate concentration is a factor that plays a role in enzyme activity. More the substrate concentration, more is the enzyme activity till it reaches saturation where even on increasing substrate will not increase the activity. Statement I agrees to it but statement IV is incomplete. Statement III is stating the presence of non-competitive inhibitor, which binds to a separate site on enzyme than the substrate and alters the active site of enzyme thus even if substrate concentration is increased it will not lead to any change. Lastly, about competitive inhibitor has a competition with the substrate for the same position of the active site. If substrate conc. is higher than it will replace competitive inhibitor for the active site and its effect on enzyme activity.
