Botany - Photosynthesis in Higher Plants Question with Solution | TestHub

Dr. Melvin Calvin discovered the correct "path of carbon" in photosynthesis, i.e. sequence of biochemical reactions of carbon dioxide fixation into glucose. He carried out experiments on unicellular green algae likeChlorella and Scenedesmus, and used radioactive isotope of carbon,${\mathrm{C}}^{14}$as a tracer.${ }^{14}\mathrm{C}{\mathrm{O}}_2$was fed to photosynthesizing algal cells for definite time in seconds.The algal cells were then killed by dropping the suspension in hot methanol.The technique of paper chromatography was used to separate the intermediates formed. He discovered that the first stable product is a 3-carbon compound, Phosphoglyceric acid (PGA). Hence Dr. Calvin called it${\mathrm{C}}_3$cycle.