Botany - Cell - The Unit of Life Question with Solution | TestHub

In order to increase the resolution, the specimen must be viewed using either shorter wavelength $(\lambda )$ light or through an imaging medium with a relatively high refractive index or with optical components which have a high numerical aperture (NA). thus, resolution of microscope in inversely proportional to the wavelength of the light used. $$\Delta d=\frac{\lambda }{2n\sin \theta }$$ Where, $$\begin{array}{rl}& \lambda =\text{ wavelength }\\ & n=\text{ refractive index }\\ & \theta =\text{ half angle subtended }\\ & \Delta d=\text{ shortest distance. }\end{array}$$