Biology - Molecular Basis of Inheritance Question with Solution | TestHub
Given below are two statements about DNA fingerprinting.
Statement (I): The DNA probe used in DNA fingerprinting is a dsDNA obtained from organisms only.
Statement (II): The DNA probe is labeled with radioisotopes and added to nitrocellulose filter paper.
In light of the above statements, choose themost appropriateanswer from the options given below.
Options:
Answer:
Solution:
Statement (I) is incorrect because the DNA probe used in DNA fingerprinting is a single-stranded DNA (ssDNA) or single-stranded RNA (ssRNA), not double-stranded DNA (dsDNA). Furthermore, probes can be artificially synthesized in the laboratory and are not exclusively obtained from organisms.
Statement (II) is correct because during the Southern blotting step of DNA fingerprinting, the single-stranded DNA fragments are transferred to a synthetic membrane such as nitrocellulose or nylon. A radiolabelled single-stranded DNA probe (VNTR probe) is then added to the nitrocellulose filter paper to hybridize with the complementary DNA sequences.
Answer: Statement (I) is incorrect but Statement (II) is correct.
