Biology - Molecular Basis of Inheritance Question with Solution | TestHub
Identify the correct order of the following enzymes used during the process of replication of eukaryotic DNA.
(A). DNA polymerase
(B). DNA ligase
(C). DNA helicase
(D). DNA gyrase
Choose thecorrectanswer from the options given below:
Options:
Answer:
Solution:
The process of DNA replication involves a coordinated sequence of enzyme actions.
DNA gyrase (acting as a topoisomerase) relieves the torsional strain and positive supercoiling generated ahead of the replication fork to facilitate the unwinding process.
DNA helicase unwinds the DNA double helix by breaking the hydrogen bonds between complementary base pairs, creating the single-stranded templates at the replication fork.
DNA polymerase catalyzes the polymerization of deoxynucleotides to synthesize the new complementary DNA strands along the templates.
DNA ligase functions at the end of the synthesis phase to seal the nicks between short segments of newly synthesized DNA, known as Okazaki fragments, on the lagging strand.
The correct sequential order of action for these enzymes is DNA gyrase (D), DNA helicase (C), DNA polymerase (A), and DNA ligase (B).
Answer: (D), (C), (A), (B).
